Investigating the Relationship between rs7903146 Polymorphism Genotypes of TCF7L2 Gene and Visfatin Serum Level in Patients with Type 2 Diabetes

Abstract

This study showed that T allele is a risk allele for both homozygous and heterozygous in rs7903146 polymorphism genotyping in TCF7L2 gene. Here, 132 individuals were investigated (87 of them were patients with type 2 diabetes and 45 other were healthy, respectively). DNA was extracted from whole blood samples via a purification kit; then in order to the determination of quality, it was electrophoresised on agarose gels. Genotyping method was performed through high-resolution melt (HRM) reverse transcription polymerase chain reaction (RT-PCR). The visfatin level was determined based on enzyme-linked immunosorbent assay (ELISA) method. According to the achieved results, increasing the plasma levels of visfatin in diabetics patients (10.73 ± 5.38) compared to the control group (6.73 ± 1.88) and their changes based on the separation of genotypes also confirms that T allele is the probable risk factor (p-value < 0.05). In addition, the presence of the T allele is related to increasing triglyceride (TG) levels of blood. Therefore, adipose tissue burning and its reduction (along with the releasing of TG in the blood) decreased the source of visfatin, therefore increasing the TG in the presence of T allele (the development of diabetes) is associated with decreased plasma levels of visfatin. According to this study, evaluating plasma levels of visfatin and TG levels in blood is useful in prognosis of type 2 diabetes.