Biofilm forming Enterococci and their Status as Emerging Multidrug Resistant Bacteria

Abstract

Urine specimens were collected aseptically and inoculated directly onto CLED and MacConkey agar medium and incubated overnight. Single target colonies were sub cultured, and identification was based on culture, morphology, and biochemical characteristics. Enterococci isolates were subjected to biofilm assay (Tube method), and resistance pattern was determined for both biofilm-formers and non-biofilm-formers. Genomic DNA was extracted using the Bioneer kit, and the gene for virulence was detected by PCR and agarose gel electrophoresis. 148 Patients having one or more urinary symptoms were the study population. The aim of the study was to determine the multidrug resistant ability of biofilm-forming Enterococci. A number of growths were encountered; Enterococci accounted for only 9.46% (14), the highest prevalence was seen in the age group 21-30 (35.71%), and females (64.29%) were more prone to enterococcal infection than their males (35.71) counterpart. There is a relationship between biofilm production and antibiotic resistance because multidrug resistant Enterococci isolate produced bands against esp gene with an average of 510kbp. Biofilm forming strains showed the highest resistance to gentamicin and penicillin (83.3%), and vancomycin can be considered a good alternative therapy in enterococcal UTIs because of its lower resistance (75%).