The Effect of Bengle (Zingiber Cassumunar Roxb.) Rhizome Chloroform Extract on Nitric Oxide and Reactive Oxygen Intermediate Secretions in Vitro

Abstract

The immune system is needed by the body to maintain its integrity against the dangers caused by various substances in the environment. Immunomodulators are substances that can regulate the immune system and increase immunity. Bangle (Zingiber cassumunar Roxb.) Provides an immunomodulatory effect with an increase in phagocytic activity of macrophages and an increase in NO and ROI. This study aims to see whether there is an increase in NO and ROI secretion on macrophage of mice in vitro. Bangle rhizome was macerated using chloroform. Qualitative analysis by TLC method. The experimental study using macrophages isolated from male Balb mice divided into three concentrated variations 25, 50, 100 μg/ml. Quantitative analysis of NO secretion levels was done by Greiss reaction assay and analysis of ROI secretion levels by NBT reduction assay. The results showed that with the administration of bangle chloroform extract concentrations of 25, 50, 100 μg/ml had a significant effect (p<0.05) on increasing NO secretion and ROI compared with the negative control group. In NO there was an increase in NO secretion levels with values in the control group, bangle chloroform extract groups concentrations of 25, 50, and 100 μg / ml respectively 11.8972μM, 17.7265μM, 19.4754μM and 22.3900μM. In ROI there was an increase in percentage ROI secretion with values in the control group, ERB groups concentrations of 25, 50, and 100 μg / ml respectively were 14.67%, 20.67%, 24%, and 27.3%. In conclusion, administration of bangle rhizome extract of with concentrations 25, 50, 100 μg/ml has significant effect (p< 0,05) to increase the percentage of ROI secretion and NO secretion level of male Balb/C mice macrophages in vitro. Keywords—bangle, immunomodulatory, NO secretion, ROI