[Evaluation of a new colorimetric test (VIP) to determine the presence of lactobacillus in saliva].

Anales de la Facultad de Odontologia Pub Date : 1990-12-01
V P Yorio, E Zinemanas
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Abstract

This techniques using Rogosa agar modificated with green of bromocresol, placed into tubes (10 ml) containing 4 ml, was developed for qualitative estimation in saliva of lactobacilli in a quick form. We spotted with a pipette 0.1 ml of saliva dilution into different tubes with the culture medium previously mentioned. The tubes were incubated at 37 degrees C for 24 and 48 hours. The results obtained showed a great agreement with the number of latobacilli in the agar.

[评价一种新的比色法(VIP)来确定唾液中乳酸菌的存在]。
本技术采用溴甲酚绿修饰的Rogosa琼脂,将其放入含有4 ml的10 ml试管中,用于快速定量测定乳酸菌唾液。我们用移液管将0.1 ml的唾液稀释到不同的管中,加入前面提到的培养基。试管在37℃下孵育24小时和48小时。所得结果与琼脂中拉托杆菌的数量基本一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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