[Analysis of cellular proteins in 9,10-dimethyl-1,2-benzanthracene induced hamster tongue carcinoma and dysplastic lesions by two-dimensional gel electrophoresis].

Abstract

The cellular proteins of hamster tongue squamous cell carcinoma and epithelial dysplastic lesions induced by 9,10-dimethyl-1,2-benzanthracend (DMBA) were analyzed by two-dimensional isoelectric focusing-sodium dodecyl sulfate gel electrophoresis. Many of the polypeptides were common to these lesions. However, there were thirteen main polypeptides which differed between normal tongue mucosa and tongue carcinoma. In the carcinoma, eight polypeptides (5.2/53 K, 5.8/47 K, 6.9/46 K, 6.9/50 K, 7.1/55 K, 7.2/46 K, 7.3/75 K, 7.4/48 K, pI/MW) were decreased and three polypeptides (4.9/32 K, 6.3/50 K, 7.2/60 K) were increased in an amount. The remaining two polypeptides (4.8/37 K, 4.9/36 K) that were not normally present were appeared in carcinoma, but these two new polypeptides were not specific to carcinoma because they were also present in dysplastic lesions. There were marked differences in the polypeptide between various degree of dysplastic lesions and carcinoma on the one hand and normal mucosa on the others. These results suggests that two-dimensional electrophoresis may have usefulness in further research on transformations from normal mucosa to malignant cancer cells.