[A study to establish the model of transseptal fibers in vitro by human gingival fibroblast].

Abstract

The present study was attempted to establish the model of transseptal fibers in vitro to clarify the remodeling mechanisms of the fibers during orthodontic treatment. Human premolars were transversally cut. The thickness of films was 150 microns and a pair of dental rings was placed at intermediate of 500 microns in tissue culture dishes. Human gingival fibroblasts were seeded in these dishes and cultured. Culturing of these cells were changed at 1, 5, 10 and 20 days and used to histological observation. The following results were obtained: 1. It was observed that human gingival fibroblasts and fibrillar materials oriented approximately the surfaces of sliced teeth and extended the process of cells along the direction from sliced teeth to another one. The bridges of cells and fibers between two sliced teeth were completed after 10 days culturing. 2. The fibrillar materials were examined by TEM and bundles constructed from banded fibrils which each band was 60 nm in diameter. The red fibers were stained with Van Gieson's stain method and so many red fibers filled in the space between the two sliced teeth. This suggests that the most of fibrillar materials were collagen fibers formed from human gingival fibroblasts. The structural materials were assumed to be resembled to transseptal fibers in vivo. This culture system is usefull to study the remodeling mechanisms of transseptal fibers in tooth movement.