[Effect of mercuric chloride poisoning on iron distribution in rats].

Abstract

The aim of our work was to establish the influence of the HgCl2 poisoning (various doses) on the Fe distribution in the rat organism. 175 Wistar rats were divided into 5 groups: I--control group, II--intoxicated with HgCl2 in the dose of 1 mg/kg of body weight, in the III group the HgCl2 dose was 6 mg/kg, in the IV group--12 mg/kg, in the V--12 mg/kg but rats of this group were given afterwards BAL (2.3-dimercaptopropanol). All rats were given 0.2 ml of 59FeCl3, radioactivity 3.7 kBq. The animals were put to sleep with chloroform after 3 and 6 hours, and 1, 2, 4, 8, 14 days after the isotope administration. For the radiometric assays the following organs or tissues were taken: stomach, small and large intestine, liver, kidneys, lungs, heart, muscles, spleen, blood, brain, testicles. The results are given in the percent of Fe dose in 1 g of wet tissue. The statistical analysis (Students' t test) was performed. The results indicate that in poisoned animals the Fe distribution was different than in controls. Increased concentration of 59Fe was noted after 3 and 6 hours in stomach and after 1 day in large intestine, whereas in other organs the Fe concentration was lower, correlating usually with the Hg dose. One interesting thing was noted: the Fe concentration in almost all organs was higher in rats intoxicated with the 12 mg/kg dose of HgCl2 and given afterwards BAL (IV group), than in rats that were not given BAL. Present results can have some practical significance in therapeutic procedure in mercury poisoning and during the convalescence.