[Effect of sodium diphenylhydantoin on sister chromatid exchange frequency in mice].

Abstract

In order to analyze the effect of the anticonvulsant diphenyl hydantoin (DPH) over the frequency of sister chromatid exchanges (SCE) in vivo, Balb-C mouse 8-12 weeks old, males and females, were injected intraperitoneally with a single dose and three different concentrations: 1, 10 and 20 mg/Kg, as negative and positive control, we used physiological serum and mitomycin-C respectively that were injected in the same way. Twenty five metaphases were analyzed per individual in the slide with the differential staining of sister chromatid, we found a light, but significantly increase, in the frequency of SCE in the concentrations of 10 and 20 mg/Kg when they were compared with the ones obtained for the negative control. In the males was observed a positive correlation in the levels of ICH when the doses of DPH were increased.