The effect of sericin application over hydroxyapatite surface on osteoblast cells proliferation

Abstract

Hydroxyapatite (HA) has been used clinically to treat bone defect. Modification of HA surface with respect to bone integration has been developed using treatments which have the potential to improve cells proliferation. Bombyx mori's sericin, a polymer protein that has polar side groups, have been known to accelerate cells attachment and proliferation. The aim of this study was to investigate the effect of sericin application over HA surface on osteoblast cells proliferation. HA discs (10 mm in diameter, 3 mm thick) were sintered. Three concentration of sericin (0.01, 0.5, and 0.1%) were applied on HA surface. Water contact angle was measured to evaluate the hydrophilicity of the disc surface. The discs were then seeded with MC3T3E1 osteoblast cells for proliferation test. The data were analyzed by Anova and LSD. Contact angle measurement showed significant increases of the hydrophilicity on sericin-modified HA surface. The amount of cells proliferation on HA discs (1.40±0.26) was significantly difference (p<0.05) with HA+sericin 0.01% (2.23±0.20), HA+sericin 0.5% (2.33±0.24), HA+sericin 0.1% (2.37±0.20). Variation of sericin concentrations applied over HA did not influence any significant difference on cells proliferation (p>0.05). The conclusion was sericin application over HA surface increased the amount of osteoblast cells proliferation. Concentration of sericin application over HA (0.01, 0.05, 0.1%) did not influence cells proliferation.