Phytochemical Screening And Antimicrobial Activity Of Hydnoraabyssinicia Root Extract

M. Ma
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Abstract

This study was carried out in Khartoum state-Sudan, during March; this plant was collected from Al-Dubibat area, locality of al-goze, South Kordofan State, western Sudan. The dried root of Hydnora abyssinicia was extracted successively with (petroleum ether, chloroform, and methanol), The phytochemical screening carried out for different plant roots extracts and showed that it contain high amount of alkaloids in all extract and moderate amount of flavonoids (in chloroform, methanol extracts) and moderate amount of tannins, sterol and triterpenes also moderate amount of cardiac glycoside and high amount of saponins. The antimicrobial activity of extracts were evaluated against four standard bacteria (Gram positive; Bacillus subtilis, Staphylococcus aureus) and (Gram negative; Escherichia coli, Pseudomonas aeruginosa).in addition of one standard fungi (Candida albicans).The result of antimicrobial tests indicated that the methanolic extract inhibited the growth of all microorganisms and most extracts showed same degree of antimicrobial activity. The result provides promising baseline information for the potential use of these crude extracts in the treatment of bacterial and fungal infec-tions.
水杉根提取物的植物化学筛选及抑菌活性研究
这项研究于3月在苏丹喀土穆州进行;该植物采自苏丹西部南科尔多凡州al-goze地区的Al-Dubibat地区。用(石油醚、氯仿、甲醇)连续提取水蛇根,对不同植物根提取物进行植物化学筛选,结果表明,水蛇根提取物中生物碱含量高,黄酮类化合物含量适中(氯仿、甲醇提取物),单宁、甾醇、三萜含量适中,心苷含量适中,皂苷含量较高。对四种标准菌(革兰氏阳性菌;枯草芽孢杆菌,金黄色葡萄球菌)和(革兰氏阴性;大肠杆菌,铜绿假单胞菌)。另外还有一种标准真菌(白色念珠菌)。抑菌试验结果表明,甲醇提取物对所有微生物的生长均有抑制作用,且大部分提取物具有相同程度的抑菌活性。该结果为这些粗提取物在治疗细菌和真菌感染方面的潜在应用提供了有希望的基线信息。
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