Identification and isolation of calcium-dependent and -independent bovine immunoglobulins reactive with sepharose 4B.

S Sugii, Y Hirota
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引用次数: 7

Abstract

Calcium-dependent and -independent Sepharose 4B-binding bovine serum proteins were isolated by affinity chromatography on unsubstituted Sepharose 4B using 2 mM ethylenediamine tetraacetic acid followed by 0.1 M galactose. They appeared in two different protein peaks by gel filtration on Sephacryl S-200. The earlier eluted protein was demonstrated to be immunologically IgM, whereas the retarded one IgG. From these findings, Sepharose 4B-binding bovine serum proteins are suggested to be calcium-dependent and -independent immunoglobulins IgM and IgG.

与蔗糖4B反应的钙依赖性和非依赖性牛免疫球蛋白的鉴定和分离。
用2 mM乙二胺四乙酸和0.1 M半乳糖在未取代的Sepharose 4B上亲和层析分离钙依赖性和非依赖性Sepharose 4B结合的牛血清蛋白。在Sephacryl S-200凝胶过滤后,它们出现在两个不同的蛋白峰上。早期洗脱的蛋白被证明是免疫IgM,而延迟的IgG。根据这些发现,Sepharose 4b结合的牛血清蛋白可能是钙依赖性和非依赖性免疫球蛋白IgM和IgG。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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