Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers

C. Herison, S. Sutjahjo, I. Sulastrini, R. Rustikawati, S. Marwiyah
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引用次数: 19

Abstract

Genetic diversity is the most important aspect in tomato breeding activities. Better assessment on the diversity of the collected accessions will come up with better result of the cultivar development. This study aimed at analyzing the genetic diversity of 27 tomato accessions by morphological and molecular markers. Twenty seven accessions collected from various regions of Indonesia were planted in the field and evaluated for their morphological traits, and RAPD analyzed for their molecular markers. The UPGMA clustering analyzes, elaborating the combination of morphological and molecular data, indicated that the tomato accessions could be grouped into 5 major groups with 70 % genetic similarity levels. Current study indicated that although many accessions came from different locations, they congregated into the same group. Cherry, Kudamati 1 and Lombok 3 were the farthest genetic distant accessions to the others. Those three genotypes will be the most valuable accessions, when they were crossed with other accessions, for designing a prospective breeding program in the future.
27份番茄材料形态和分子标记遗传多样性分析
遗传多样性是番茄育种活动中最重要的方面。对选育材料的多样性进行更好的评价,有利于品种的发展。利用形态和分子标记分析了27份番茄材料的遗传多样性。选用印度尼西亚不同地区的27份材料进行田间种植,对其形态性状进行评价,并对其分子标记进行RAPD分析。UPGMA聚类分析结合形态学和分子数据,表明番茄材料可分为5个主要类群,遗传相似性水平为70%。目前的研究表明,虽然许多物种来自不同的地点,但它们聚集在同一个群体中。Cherry、Kudamati 1号和龙目岛3号是其他品种遗传距离最远的亲本。这3个基因型将是最有价值的材料,当它们与其他材料杂交时,将为设计未来有前景的育种计划提供依据。
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