IMPROVEMENT OF THE CONTENT OF THE DRIGALSKI LACTOSE AGAR NUTRIENT MEDIUM

Abstract

The purpose of the study is to improve the mix formulation of the Drigalski Lactose Agar breeding ground, produced by AppliChem, and aimed at enterobacteria isolation and differentiation. Development of a new mix formulation for the Drigalski Lactose Agar breeding ground, efficient assessment of cultivation of enterobacteria on its improved base and other breeding grounds used in the laboratory practice by the authors was the task of the study. The im-proved agar Drigalsky differential medium with lactose is recommended for cultivation (isolation) of different enter-obacteria of Enterobacteriaceae family.Differentiation of enterobacteria on improved medium is carried out accord-ing to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The medium can also be used for sanitary and microbiological examination of environmental objects.The medium can be used also to conduct the ONPG test. The efficiency of cultivation of enterobacteria isolated from various animal species on the most frequently used differential diagnostic medium, including new formulation of the Drigalski Lactose Agar, ranged from 16.28 ± 1.44 to 42.18 ± 4.12 hours. Enterobacteria isolated from farm and wild animals formed colo-nies on improved Drygalsky agar with lactose during 24 hours, and enterobacteria isolated from zoo animals within 25-31 hours.