Light field Fourier ptychographic microscopy

Abstract

In this paper, a novel microscopic imaging technology, termed light field Fourier ptychographic microscopy (LFFPM), is presented which enables the reconstruction of wide-field, high-resolution images with the depth information by utilizing a light-emitting-diode (LED) illumination matrix.Conventional light field microscope lacks the ability of high lateral resolution because it sacrifices its lateral resolution to its angular resolution. Compared to conventional light field microscope, LFFPM enhances the lateral resolution with no need for the object to be in focus. Using LFFPM, the result shows that the lateral resolution is more than 2 times better than light field microscopy in simulation and 1.5 times in the experiment. Furthermore, the simulation result also shows that the ability of LFFPM to recovery the information of the phase and amplitude.Thus, this technology has a wide prospect of various biomedical applications such as neuroanatomy and haematology.