ASSESSMENT OF TOLERANCE OF MICROALGAE TO DIFFERENT TEMPERATURE REGIMES

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Abstract

Today there is a serious environmental problem - global climate change, and one of the main reasons for this change is the release of so-called greenhouse gases, and primarily raw carbon dioxide. One strategy to reduce the concentration of carbon dioxide in the atmosphere is to capture carbon dioxide from industrial emissions, since this is where the gas is found in high concentrations. In addition to physical and chemical methods, carbon dioxide capture is possible with the help of biological methods, for example, using microalgae. However, the optimal growth temperatures for microalgae are in the range of 20-30 degrees Celsius, and the emission temperature is much higher. In general, heat-resistant species can be distinguished, for example, from hot springs. But, as a rule, such species require specific growing conditions, which on an industrial scale will lead to an increase in the cost of equipment. The purpose of this work was to carry out adaptation to semi-high temperatures of microalgae species selected from local conditions. We conducted an experiment in which we compared the growth of three types of green microalgae isolates at room temperature and elevated temperature. The isolates were used in their initial state and after adaptation at gradually elevated temperatures. It was demonstrated that temperature adoption led to higher yield of the isolates when they were cultivated at 36 -C. Thus, for strain 2 the Dopt of the previously adapted variant 2e was 1.9-fold higher as compared with that of the initial variant 2i. For the other two strains such a difference was less, but still significant. The results obtained demonstrate the potential of the green algal species to be adopted to elevated temperature which is important for their future use in the biotechnologies of carbon capturing from the industrial emissions.
微藻对不同温度环境的耐受性评估
今天有一个严重的环境问题——全球气候变化,而造成这种变化的主要原因之一是所谓的温室气体的释放,主要是原始的二氧化碳。减少大气中二氧化碳浓度的一个策略是捕获工业排放的二氧化碳,因为工业排放的二氧化碳浓度很高。除了物理和化学方法之外,利用生物方法捕获二氧化碳也是可能的,例如利用微藻。然而,微藻的最佳生长温度在20-30℃范围内,其排放温度要高得多。一般来说,耐热物种可以从温泉中区分出来。但是,一般来说,这些物种需要特定的生长条件,这在工业规模上将导致设备成本的增加。本研究的目的是从当地条件中选择微藻物种进行半高温适应。我们进行了一项实验,比较了三种绿色微藻分离物在室温和高温下的生长情况。分离株在初始状态下使用,并在逐渐升高的温度下适应。结果表明,在36℃的温度下培养,菌株的产量较高。因此,对于菌株2,先前适应的变体2e的采用率比初始变体2i高1.9倍。对于其他两个菌株,这种差异较小,但仍然显著。研究结果表明,绿藻具有适应高温环境的潜力,这对它们未来在工业排放碳捕获生物技术中的应用具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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