Occurrence and biotechnological potential of yeasts associated with almond fruits of Attalea Speciosa mart ex Spreng / Ocorrência e potencial biotecnológico de leveduras associadas aos frutos de Attalea speciosa.
Stella Costa Santos do Vale, A. P. Guimarães, P. B. Morais
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引用次数: 0
Abstract
Attalea speciosa Mart ex Spreng is a oil palm belonging to the family Arecaceae with fruits 15% of the total weight of the fruit correspond to the epicarp; 20% to 58% to the mesocarp and endocarp (including almonds), the almonds being its most used component. Fruits are important natural microhabitats for a significant diversity of yeast species. The objective of this study was to isolate and test the enzymatic abilities of yeast strains associated with almond fruits of Attalea speciosa, and their possible biotechnological potential for industrial purposes. Ten collections of 15 almonds for sample were conducted at seven days intervals at an extractivism reserve of Mata de Babacuais ecosystem. Among 84 yeasts isolated, The enzymatic activity was evaluated and among the eighty-four strains of yeast tested as the ability to hydrolyze casein among protease, 26.04% had enzymatic potential acidic pH, 31.92% at neutral pH and 28.56% in alkaline pH, demonstrating enzyme potential in different pHs.A lipase activity was also satisfactory where, sixty-two lipase producing strains may have potential use for commercial employment testing microbial lipases. Already cellulolytic activity was more limited, only nineteen lineages showed degrading cellulose.
Attalea speciosa Mart ex春是一种油棕,属于槟榔科,果实占果实总重量的15%,对应于外果皮;中果皮和内果皮(包括杏仁)占20%至58%,杏仁是其最常用的成分。果实是酵母种类多样性的重要天然微生境。本研究的目的是分离和测试与Attalea speciosa杏仁果实相关的酵母菌株的酶促能力,以及它们在工业上可能的生物技术潜力。在Babacuais Mata de Babacuais生态系统的提取保护区内,每隔7天采集10个杏仁样品,共15个。对84株酵母菌进行酶活性评价,84株酵母菌在蛋白酶中水解酪蛋白的能力测试中,酸性pH值为26.04%,中性pH值为31.92%,碱性pH值为28.56%,显示出不同pH值下的酶势。脂肪酶活性也令人满意,其中62株脂肪酶产生菌株可能有潜在的商业用途,用于测试微生物脂肪酶。纤维素降解活性已经很有限,只有19个谱系表现出降解纤维素的能力。