[Experimental studies of extracellular matrix constituents for coating artificial prostheses before endothelialization].

M Kadletz, K Wolfrum, M Grimm, M Müller, E Wolner
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Abstract

Endothelialization seems to be a possibility to reduce thrombogenicity of artificial vascular prostheses. We have been occupied to find an ideal coating for the cells. Substances of extracellular matrix (ECM) were used: human fibronectin (HFN), laminin, collagen type I + III + IV. We have compared the influence of these materials with regard to adherence, sheer stress resistance and growth of cultivated human vena saphena endothelial cells (SEC). There was not any statistically significant difference according to cell proliferation. During a cultivation period of 7 days SEC increased by population doubling number 5 corresponding to a population doubling time of 76 hours. PTFE prostheses were coated with the materials, seeded with SEC and exposed to sheer stress in a physiological perfusion unit. Adherent SEC were counted after 24 hours of perfusion. Counts were correlated to the number of seeded cells. After perfusion only 23 +/- 4%, resp. 25 +/- 6% of cells were counted on collagen I + IV + HFN and on collagen I + IV + laminin. We found better results on collagen I + III and on collagen I + III + HFN; there were adherent 55 +/- 5%, resp. 53 +/- 7% after perfusion. These results document that there is no difference for SEC cultivation on different native components of basal membrane, under sheer stress conditions there seems to be differences in cell adherence.

人工假体内皮化前涂层细胞外基质成分的实验研究
内皮化似乎有可能降低人工血管假体的血栓形成性。我们一直在忙着为电池寻找一种理想的涂层。使用细胞外基质(ECM)物质:人纤维连接蛋白(HFN)、层粘连蛋白、I + III + IV型胶原。我们比较了这些材料对培养的人隐静脉内皮细胞(SEC)的粘附性、绝对抗逆性和生长的影响。细胞增殖差异无统计学意义。在7天的培养期间,SEC增加了种群倍增数5,对应于种群倍增时间为76小时。聚四氟乙烯假体涂上材料,用SEC播种,并在生理灌注单元中暴露于绝对应力下。灌注24h后,计数粘附体SEC。计数与种子细胞的数量相关。灌注后仅23 +/- 4%。胶原I + IV + HFN和胶原I + IV +层粘连蛋白占25 +/- 6%。我们发现胶原I + III和胶原I + III + HFN效果更好;贴壁55±5%;53 +/- 7%灌注后。这些结果表明,在不同的天然基膜组分上,SEC培养没有差异,在完全胁迫条件下,细胞粘附似乎存在差异。
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