Antibiotic resistance and virulence characterization of Pseudomonas aeruginosa strains isolated from respiratory tract infections

Medycyna doświadczalna i mikrobiologia Pub Date : 1900-01-01 DOI:10.32394/mdm.72.03

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Abstract

Objectives: The aim of this study was to analyze the resistance, virulence and biofilm production of PA strains from respiratory tract infections. Methods: All strains were tested for antibiotic susceptibility according to current EUCAST guidelines. Several resistance and virulence genes were detected by PCR. Biofilm formation was detected by the crystal violet method. Results: 38 strains (47.5%) were categorized as XDR, and 12 (15%) as MDR. Seven (10%) MBL producers were identified. The VIM gene was detected in two strains. Genes connected with aminoglycoside resistance: aac(6΄)-I and ant(2΄΄)-I were detected in 10 strains each, aac(6′)-Ib in 18 strains (21%), and aph (3’)-IV in 7 strains (8%). The most prevalent virulence genes were exoT (95%), apr (93%), A, phzS (95%), and plcH (95%). All the tested strains were strong biofilm producers. Conclusions: The results demonstrate high frequency and heterogeneity of virulence gene profiles among PA isolates from respiratory tract infections. A high number of MDR and XDR strains poses a serious threat for patients and shows clearly that new antimicrobial agents are needed.

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呼吸道感染铜绿假单胞菌菌株的耐药性和毒力分析

目的:分析呼吸道感染PA菌株的耐药性、毒力和生物膜生成情况。方法:按照现行EUCAST指南对所有菌株进行药敏试验。PCR检测到多个抗性和毒力基因。用结晶紫法检测生物膜的形成。结果:38株(47.5%)为XDR, 12株(15%)为MDR。确定了7个(10%)MBL生产商。在两株菌株中检测到VIM基因。与氨基糖苷耐药相关的基因:aac(6′)-I和ant(2΄΄)-I各10株，aac(6′)-Ib 18株(21%)，aph(3′)-IV 7株(8%)。最常见的毒力基因为exoT(95%)、apr(93%)、A、phzS(95%)和plcH(95%)。所有被试菌株均具有较强的生膜能力。结论:从呼吸道感染中分离出的PA毒力基因谱具有较高的频率和异质性。大量耐多药和广泛耐药菌株对患者构成严重威胁，并清楚地表明需要新的抗微生物药物。

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Medycyna doświadczalna i mikrobiologia

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