Antioxidant Activity of Methanol Extract from Several Indonesian Green Teas

A. Simamora, M. Steven, A. Santoso, Flora Rumiati, K. Timotius
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Abstract

As a common beverage, green tea is supposed to have beneficial health effect, such as antioxidant. At present, there are many green teas available in the market in Jakarta. Their quality, in their antioxidant activity, should be proved. This study aimed to investigate the antioxidant activity of extracts of green tea products commercially available in Jakarta. Four green tea samples from different factories (coded as A, B, C, and D) were selected and macerated using methanol. DPPH radical scavenging activity, reducing power ability and total antioxidant capacity were used to measure the antioxidant activity. The total phenolic content (TPC) was also determined. The studied green teas had varied TPC from 23.80  to  84.03 mG GAE/g extract, ranked as B > D > A > C. All samples exhibited various but strong antioxidant activity by DPPH assay, even better than standards ascorbic acid and butylated hydroxytoluene (BHT). However, all extracts showed similar activities in their reducing power ability and total antioxidant capacity, with activities less than standards. These findings confirm that the quality of the Indonesian commercial green teas were heterogeneous both in TPC or DPPH scavenging capacity. But, there is an indication that they are good as an antioxidant containing beverage.Â
几种印尼绿茶甲醇提取物的抗氧化活性研究
作为一种常见的饮料,绿茶被认为具有抗氧化等有益健康的作用。目前,雅加达市场上有很多绿茶可供选择。其抗氧化活性的质量有待证实。本研究旨在探讨雅加达市售绿茶产品提取物的抗氧化活性。从不同的工厂(编号为A、B、C和D)选择四种绿茶样品,用甲醇浸泡。以DPPH自由基清除能力、还原能力和总抗氧化能力为指标测定其抗氧化活性。测定了总酚含量(TPC)。所研究绿茶的TPC值为23.80 Â ~ Â 84.03 mG GAE/g提取物,按B > D > A > c顺序排列。DPPH测定结果显示,所有样品均表现出不同但较强的抗氧化活性,甚至优于标准抗坏血酸和丁基羟基甲苯(BHT)。但各提取物的还原能力和总抗氧化能力相近,均低于标准。这些发现证实了印度尼西亚商业绿茶的质量在TPC或DPPH清除能力方面存在异质性。但是,有迹象表明它们是一种很好的抗氧化剂,含有beverage.Â
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