Chromatin fluorescence after carmine staining.

J C Stockert, A R Llorente, P Del Castillo, A Gómez
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引用次数: 8

Abstract

After staining with dilute solutions (0.1 mg/ml in distilled water) of commercial carmine, a strong reddish orange fluorescence was observed in nuclei from cell smears and frozen and paraffin tissue sections. Optimal exciting light was 436 nm (violet-blue) or 450-490 nm (blue). Compact chromatin from interphase nuclei, mitotic and meiotic chromosomes and the kinetoplast of Trypanosoma cruzi showed the highest fluorescence, while the basophilic cytoplasm appeared weakly fluorescent. No emission was observed in cartilage matrix, mast cell granules or goblet cell mucin. This selective method could be valuable in microscopic and cytochemical studies on chromatin because the carmine fluorescence is stable and preparations can be dehydrated and mounted permanently without changes in the fluorescence pattern.

胭脂红染色后的染色质荧光。
用商业胭脂红稀释溶液(0.1 mg/ml蒸馏水)染色后,在细胞涂片、冷冻和石蜡组织切片的细胞核中观察到强烈的红橙色荧光。最佳激发光为436 nm(紫蓝色)或450-490 nm(蓝色)。克氏锥虫间期核致密染色质、有丝分裂和减数分裂染色体和着丝质体的荧光最强,而嗜碱性细胞质的荧光较弱。软骨基质、肥大细胞颗粒、杯状细胞黏蛋白均未见释放。这种选择性方法在染色质的显微镜和细胞化学研究中是有价值的,因为胭脂红荧光是稳定的,并且制备物可以脱水和永久安装而不会改变荧光模式。
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