Cannulation and continuous cross-sectional area measurement of small blood vessels

Journal of pharmacological methods Pub Date : 1990-11-01 DOI:10.1016/0160-5402(90)90032-G

Ed Vanbavel, Trudi Mooij, Maurice J.M.M. Giezeman, Jos A.E. Spaan

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引用次数: 32

Abstract

Techniques have been developed for the study of isolated small arteries. To pressurize and perfuse segments of these vessels, a cannula with a low resistance to flow was developed. This cannula consisted of two concentric micropipettes. The end of a vessel segment was sucked into the inner pipette and clamped by applying subatmospheric pressure on the outer pipette. Subsequently, the vessel was pressurized via the inner pipette. To enable perfusion, the segment was cannulated at both ends.

Mean cross-sectional area (CSA) of the cannulated segments was continuously measured using a fluorescence technique. The emission of light by fluorescein isothiocyanate (FITC) labeled dextran in the vessel lumen was measured using a photomultiplier tube (PMT). PMT current was linearly related to the vessel CSA.

Twenty-nine rat mesenteric vessels with inside diameters ranging from 110 to 350 μm (mean 226 μm) when maximally dilated at 80 mm Hg were cannulated. CSA was monitored during variations in perfusion pressure and addition of vasoactive agents.

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小血管插管和连续横截面积测量

研究分离小动脉的技术已经发展起来。为了对这些血管段加压和灌注，开发了一种低流动阻力的套管。该套管由两个同心微移液管组成。容器段的末端被吸入内移液器，并通过在外移液器上施加大气压来夹紧。随后，通过内移液管对容器加压。为了使灌注，在节段两端插管。利用荧光技术连续测量中空节段的平均横截面积(CSA)。利用光电倍增管(PMT)测量了异硫氰酸荧光素(FITC)标记的葡聚糖在血管腔内的发光。PMT电流与血管CSA呈线性相关。29只大鼠肠系膜血管在80 mm Hg下最大扩张时，内径为110 ~ 350 μm(平均226 μm)。在灌注压力变化和添加血管活性药物期间监测CSA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of pharmacological methods

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