PHOTOSYNTHETIC PIGMENT CONTENT AS A CONSEQUENTAL RESISTANCE SIGN OF POTATO VARIETIES TO HIGH TEMPERATURE AND MOISTURE LACK

A. L. Bakunov, Бакунов Алексей Львович, A. Milekhin, Милехин Алексей Викторович, S. Rubtsov, Рубцов Сергей Леонидович, S. N. Shevchenko, Шевченко Сергей Николаевич
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引用次数: 1

Abstract

The aim of the research is increasing potato yield in dry conditions of the Middle Volga region. The research was carried out on the experimental field of the Samara Scientific Research Institute of Agriculture – branch of the Sa-mara Research Center of the Russian Academy of Sciences. The variety testing plant nursery included 28 varieties of potatoes of domestic selection. Udacha, Zhigulevsky, Arosa and Gala breeds were the standard varieties. The plant material was planted out in two replications with 25 plants in each. To determine the content of photosynthetic pigments, extracts were prepared from ten potato leaves of each breed in 100% acetone. Measurements were per-formed by spectrophotometry. To measure the amount of chlorophyll a, the wavelength of 665 nm, chlorophyll b – 649 nm, and carotenoids – 440 nm were used. The concentration of pigments was determined by using Wettstein formulas. When harvesting, the potato yield was taken into account. The average concentration of chlorophyll a in the studied breeds was 0.92 mg/g, the average concentration of chlorophyll b was 0.62 mg/g, and one of carote-noids was 0.27 mg/g. Potato breeds with high concentration of photosynthetic pigments were identified. A reliable average dependence of the yield of potato breeds on the concentration of chlorophyll b in plants was revealed. The correlation coefficient was 0.42. There is no reliable dependence tested of yield on the concentration of chlorophyll a and carotenoids. Potato productivity is associated with a significant negative relationship with the ratio of both a and b chlorophyll concentrations. The most highly productive varieties were characterized by a high content of two varieties of chlorophyll. A high concentration of chlorophyll b or a minimum ratio of chlorophyll a and b concentra-tions can be tentatively recommended as a consequential sign for identifying potato breeds that are highly adapted to high air temperature and insufficient moisture.
光合色素含量是马铃薯品种抗高温和缺水的重要标志
这项研究的目的是提高伏尔加河中部地区干旱条件下的马铃薯产量。这项研究是在俄罗斯科学院萨马拉研究中心萨马拉农业科学研究所的试验场进行的。品种试验苗圃共选育国产马铃薯28个品种。乌达查、日格列夫斯基、阿罗萨和加拉是标准品种。植物材料分两个重复种植,每个重复25株。为测定光合色素的含量,采用100%丙酮提取10个马铃薯叶片。采用分光光度法测定。测定叶绿素a的波长为665 nm,叶绿素b - 649 nm,类胡萝卜素- 440 nm。采用韦特斯坦公式测定颜料的浓度。在收获时,马铃薯的产量被考虑在内。叶绿素a平均浓度为0.92 mg/g,叶绿素b平均浓度为0.62 mg/g,类胡萝卜素平均浓度为0.27 mg/g。鉴定出光合色素含量较高的马铃薯品种。揭示了马铃薯品种产量与植株叶绿素b浓度的平均依赖关系。相关系数为0.42。叶绿素a和类胡萝卜素的浓度对产量没有可靠的依赖关系。马铃薯产量与a和b叶绿素浓度的比值呈显著负相关。高产品种的特点是两个品种的叶绿素含量高。可以初步推荐叶绿素b的高浓度或叶绿素A和b浓度的最小比例作为识别高度适应高温和水分不足的马铃薯品种的相应标志。
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