First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo

Livestock Studies Pub Date : 2021-09-22 DOI:10.46897/livestockstudies.610203

Fırat Korkmaz, I. Bastan, D. Şahi̇n, Seher Simsek, M. Satilmis

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引用次数: 1

Abstract

Conventional buffalo semen freezing studies are limited in Anatolian buffaloes, which are overly sensitive to exogenous stimulation. The present study's object was to determine the main features of Anatolian Buffalo semen obtained by artificial vagina method for the first time. A total number of 150 ejaculates were collected from three Anatolian Buffalo bulls (app. 4 years of age). The mean pH, volume and concentration of semen were found 6.63±0.15, 1.61±0.5 ml, 1629±222.67 x10 6 spermatozoa/ml, respectively. The sperm motion characteristics were determined by using a computer-assisted sperm analysis system (CASA); the total and progressively motile sperm values were 57.12±5.63%, 23.22±4.47% and other kinetic parameters such VAP, VSL, VCL, ALH, BCF, STR, LIN were found 94.71±8.48 µm/s, 72.6±7.08 µm/s, 160.9±15.66 µm/s, 7.8±3.75 µm, 29.15±1.56 Hz, 76.91±3.87%, 46.21±2.61%, respectively after thawing. Among buffalo bulls, differences in semen pH values were statistically significant (P<0.05), while differences in ejaculate volume, semen concentration, total motility, progressive motility, VAP, VSL, VCL, ALH, BCF, STR, and LIN

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安纳托利亚水牛首次商业精液冷冻保存及主要精子学特征

传统的水牛精液冷冻研究仅限于安纳托利亚水牛，它们对外源刺激过于敏感。本研究的目的是首次确定用人工阴道法获得的安纳托利亚水牛精液的主要特征。从三只安纳托利亚水牛公牛(约4岁)中收集了总共150次射精。精液的平均pH值为6.63±0.15 ml，体积为1.61±0.5 ml，浓度为1629±222.67 × 10个精子/ml。采用计算机辅助精子分析系统(CASA)测定精子运动特征;精子总运动值为57.12±5.63%，渐进式运动值为23.22±4.47%，其他动力学参数VAP、VSL、VCL、ALH、BCF、STR、LIN分别为94.71±8.48µm/s、72.6±7.08µm/s、160.9±15.66µm/s、7.8±3.75µm、29.15±1.56 Hz、76.91±3.87%、46.21±2.61%。在水牛公牛中，精液pH值的差异有统计学意义(P<0.05)，而射精量、精液浓度、总运动性、进行性运动性、VAP、VSL、VCL、ALH、BCF、STR和LIN的差异有统计学意义

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Livestock Studies

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