Potential use of Algae Microcystis aeruginosa (Chroococaceae) in bioremediation

O. Akoma, W. N. Chris-Iwuru
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Abstract

A comparison of growth response of algal species in varying concentrations of petroleum products to assess their bioremediation potentials was carried out using Microcystis aeruginosa as test organism. The modified Chu #10 standard medium for algal culture was used for the experimental set up which lasted for twenty-one days. The test alga was subjected to growth medium with varying concentrations of petrol and kerosene. Algal growth was determined by measuring optical density of inoculated medium at three days’ interval using a spectrophotometer at 750nm wavelength. The effect of the hydrocarbons on algal growth was either stimulatory or inhibitory depending on the concentration. The inhibitory effects of the hydrocarbons increased with increasing concentrations. Comparatively, petrol had more inhibitory effect than kerosene which did not appreciably alter growth either at lower or higher concentrations. The experiment concludes that Microcystis aeruginosa can be used for bioremediation of soil or water impacted by crude oil or petroleum products. Keywords: Algal culture, Petroleum hydrocarbons, Bioremediation, Microcystis aeruginosa
铜绿微囊藻在生物修复中的潜在应用
以铜绿微囊藻为试验生物,比较了不同浓度石油产品中藻类的生长响应,以评估其生物修复能力。实验设置采用改良的楚10号标准藻培养基,持续21 d。试验藻类被置于含有不同浓度汽油和煤油的培养基中。用750nm波长分光光度计测定接种培养基的光密度,每隔三天测定一次藻类的生长情况。烃类化合物对藻类生长的影响根据浓度的不同表现为刺激或抑制。烃类化合物的抑制作用随着浓度的增加而增强。相比之下,汽油比煤油具有更强的抑制作用,在较低或较高浓度下,煤油对生长都没有明显的影响。试验结果表明,铜绿微囊藻可用于原油或石油产品污染的土壤或水体的生物修复。关键词:海藻培养,石油烃,生物修复,铜绿微囊藻
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