PROTECTIVE EFFECT OF GREEN TEA EXTRACT ON OVARY TISSUE FUNCTION IN RATS TREATED BY MALATHIONIN SECTICIDE

Journal of Jahrom University of Medical Sciences Pub Date : 2015-10-15 DOI:10.29252/JMJ.13.3.52

Hoseini Shahrkhafri, V. Hemayatkhahjahromi, E. S. Jahromi

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引用次数: 2

Abstract

Introduction: This study was conducted to investigate the protective effect of green tea extract on ovary tissue function in rats treated by Malathion insecticide. Materials and Methods: A total of 72 mature Wistar rats aged 2 to 3 months (approximate weight of 200±15 g) were experimented. Rats were divided into 9 groups of eight. Control group did not receive any medication. Sham group was given 0.2 cc physiological serum and experimental groups 1, 2 and 3 received 100, 200 and 400 mg/KG bw green tea extract respectively, experimental 4 was given 40 mg/KG bw Malathion and experimental groups 5, 6 and 7 received 100, 200 and 400 mg/KG bw green tea extract respectively and 40 mg/KG bw Malathion. After 15 days, the serum levels of sex hormones were measured, and ovaries were removed for counting the ovarian follicles. Results: Serum concentration of estrogen, progesterone, FSH and LH were significantly decreased in experimental group 4 compared to control and other groups. The primary and Graafian follicles decreased in experimental group 4 compared to control group. Primary and secondary follicles and corpus luteum significantly decreased in experimental group 4 compared to control group. All examined parameters except Graafian follicles and corpus luteum increased in the experimental groups 5, 6 and 7 compared to experimental group 4. Follicular atresia increased in experimental group 4 compared to other groups and significantly decreased in experimental groups 5, 6 and 7 compared to experimental group 4. Conclusions: Malathion had an adverse effect on secretion of sex hormones in female rats, as well as the process of oogenesis, and green tea extract decreased those negative effects.

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绿茶提取物对马拉硫磷杀虫剂处理大鼠卵巢组织功能的保护作用

前言:本研究旨在探讨绿茶提取物对马拉硫磷杀虫剂处理后大鼠卵巢组织功能的保护作用。材料与方法:选用2 ~ 3月龄Wistar大鼠72只，体重约200±15 g。大鼠被分成9组，每组8只。对照组不给予任何药物治疗。假手术组给予0.2 cc生理血清，实验1、2、3组分别给予100、200、400 mg/KG bw绿茶提取物，实验4组分别给予40 mg/KG bw马拉硫磷，实验5、6、7组分别给予100、200、400 mg/KG bw绿茶提取物和40 mg/KG bw马拉硫磷。15天后，测定血清性激素水平，并切除卵巢计数卵泡。结果:实验4组血清雌激素、孕酮、卵泡刺激素、LH浓度较对照组和其他组明显降低。实验组4与对照组相比，原代卵泡和霰粒卵泡减少。实验4组原发性、继发性卵泡和黄体较对照组明显减少。实验5、6、7组除毛囊和黄体外，其他指标均较实验4组增加。实验4组卵泡闭锁发生率较其他组升高，实验5、6、7组较实验4组显著降低。结论:马拉硫磷对雌性大鼠性激素分泌及卵发生过程有不利影响，绿茶提取物可减轻这些不利影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Jahrom University of Medical Sciences

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