Biostatistical analysis on incidence of bacteria

Abstract

This study investigated the incidence of bacteria and their antibiogram profile urine samples collected from students of the Northwest A&F University, Yangling, Shaanxi, China. A total of thirty (30) midstream urine samples were collected and bacteria in the samples were isolated using the spread plate method after which biochemical characterization was carried out. Escherichia coli, Pseudomonas spp , Klebsiella species , Salmonella species and Proteus species were the bacterial species isolated after which Kirby-Baurer disc diffusion antimicrobial assay was used to test their sensitivity against various antibiotics, such as Gentamycin, Cotrimozaxole, Ceftriaxone, Amoxyclay, Levofloxacin and Clavatin. From the study, Proteus spp shows zones of inhibition of (7mm for Cotrimozaxole), (5mm for Ceftriaxone), 5mm (for Gentmycin), (5mm), and 4mm (for Amoxyclav). E. coli 6.2 mm (Gentamycin), 3.6 mm (Levofloxacin), and 3.6 mm (Levofloxacin) zones of inhibition respectively . Gentamycin obtained 4.00mm, Levofloxacin 2.00mm and erythromycin 1.00 mm against Pseudomonas spp . Zones of inhibition were only observed on Clavatin (2.1 mm) for Salmonella species, while Klebsiella species was however , resistance to all the antibiotics tested . The highest sensitivity was observed in Cotrimozaxole which obtained a diameter of 7mm for Proteus spp ., while the lowest zone was recorded for Pseudomonas species against Erythromycin with a diameter of 1 mm. This study revealed that most of the isolates were resistant to the antimicrobial agents the implication of antibacterial resistance as shown in the study. This study therefore recommends that proper hygiene should be observed before ingestion of food and water.