I. Kraievska, Z. Tkachuk, I. Vagina, G. Gerashchenko, T. Yakovenko, V. Kashuba
{"title":"The influence of oligoribonucleotide complexes with D-mannitol on tumor formation in a murine model of B16 melanoma","authors":"I. Kraievska, Z. Tkachuk, I. Vagina, G. Gerashchenko, T. Yakovenko, V. Kashuba","doi":"10.3390/ecmc2019-06329","DOIUrl":null,"url":null,"abstract":"One of the ways of effective treatment of melanoma is based on strategies to enhance the body's immune response. OligoribonucleotidesD-mannitol (ORNs-D-M) complexes possess immunotherapeutic actions due to the activation of immune responses. Adult male mice of a C57BL/6J line were injected once by the ORNs-D-M solution at concentrations of 1.4; 0.7; 0.35; 0.175 mg. In the group where animals received 1.4 mg of ORNs-D-M, the formation of the solid tumors was not observed; however, in the group with 0.7 mg dose, the average tumor volume was 97% lower than the non-drug group. Also, the mRNA expression levels of markers of T-cell counts CD3, CD4, CD8, and CD247 approached those of healthy animals. Immunosuppressive state was decreased which reflect in the increased expression of CD274, PDCD1, and IFNB1.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"37 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/ecmc2019-06329","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
One of the ways of effective treatment of melanoma is based on strategies to enhance the body's immune response. OligoribonucleotidesD-mannitol (ORNs-D-M) complexes possess immunotherapeutic actions due to the activation of immune responses. Adult male mice of a C57BL/6J line were injected once by the ORNs-D-M solution at concentrations of 1.4; 0.7; 0.35; 0.175 mg. In the group where animals received 1.4 mg of ORNs-D-M, the formation of the solid tumors was not observed; however, in the group with 0.7 mg dose, the average tumor volume was 97% lower than the non-drug group. Also, the mRNA expression levels of markers of T-cell counts CD3, CD4, CD8, and CD247 approached those of healthy animals. Immunosuppressive state was decreased which reflect in the increased expression of CD274, PDCD1, and IFNB1.