Identifying Taxonomic Belonging of Animal HairUsing Instrumental and Microscopic Methods

L. Derecha, Oleksandr Borzov, Florin Rusitorіu
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Abstract

Urgent need to develop a methodology for identifying taxonomic belonging of animal hair using modern research methods (identification of additional objective criteria in the case of a comparative research on material evidence, namely: animal hair) is considered that contributes to solving a larger range of issues raised while appointment forensic biological examination. Authors aimed to substantiate possibility of identifying the taxonomic affiliation of animal hair using instrumental and microscopic methods, in particular, scanning electron microscope, allowing forensic biologists to study the surfaces of objects of animal origin, both of significant linear dimensions, and of microparticles in a wide range of magnifications (from 10× to more than 200,000×), providing a resolution of up to 10–9 m. Obtaining a general image of the surface and selecting appropriate areas for detailing (at the maximum possible magnifications) does not require significant reconfiguration of the device. A significant depth of focus provides detailed images of various surfaces with developed relief (cracks; traces of mechanical damage; morphological specifics of periodically reproduced surface structures). Contrast is the detection result of reflected electrons (depending on the distribution of phase components differing in chemical composition the studied sample surface). The proposed method of researching animal hair will significantly expand possibilities of examination of these objects: it will reduce the time and increase the accuracy of conducting such research, as well as make it possible to supplement illustrative material (for example, microphotographs of hair cuticles of frequently encountered animal species).
用仪器和显微镜方法鉴定动物毛发的分类归属
人们认为,迫切需要开发一种利用现代研究方法识别动物毛发分类学归属的方法(在物证的比较研究中确定额外的客观标准,即:动物毛发),这有助于解决在指派法医生物学检查时提出的更大范围的问题。作者旨在证实使用仪器和显微镜方法(特别是扫描电子显微镜)识别动物毛发分类关系的可能性,使法医生物学家能够研究动物起源物体的表面,无论是显着的线性尺寸,还是在广泛的放大范围内(从10倍到超过20万倍)的微粒,提供高达10-9米的分辨率。获得表面的一般图像并选择适当的区域进行详细描述(在最大可能的放大倍率下)不需要对设备进行重大的重新配置。重要的聚焦深度提供了各种表面的详细图像,这些表面具有发达的浮雕(裂缝;机械损伤痕迹;周期性再现的表面结构的形态特征)。对比是反射电子的检测结果(取决于所研究样品表面化学成分不同的相组分的分布)。所提出的研究动物毛发的方法将大大扩大对这些物体进行检查的可能性:它将减少进行此类研究的时间并提高其准确性,并使补充说明性材料成为可能(例如,经常遇到的动物物种的毛发角质层的显微照片)。
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