Histopathological Study in Stomach and Intestine of Anabas testudineus (Bloch, 1792) under Almix Exposure

Abstract

The aim of the present study was to investigate the histopathological alterations in the stomach and intestine of Indian freshwater teleost, Anabas testudineus (Bloch, 1792) after Almix® exposure both under laboratory and field conditions. The field (dose 8 g/acre) and laboratory (dose 66.67 mg/l) experiments was carried out for 30 days. Special type of cage was prepared and installed in the pond for the field experiment. Pathological alterations in the concerned fish organs namely stomach and intestine were assessed through light microscopy, scanning and transmission electron microscopy. Lesions observed under light microscopy also endorsed the findings of ultrastructural observations both under laboratory and field conditions. Cytopathological alterations observed under light and electron microscopy revealed that the degree of responses were different in different fish tissues as well as under conditions, here in particular effects in stomach were more prominent in laboratory condition. The overall responses registered in the fish tissues under laboratory condition were more pronounced than field condition. Therefore, these symptoms and/or alterations in the present study due to almix intoxication could be considered as biomarkers in toxicity study in aquatic ecosystem.