Phytochemical Profiling and GC-MS Analysis of Extracts of Two Tropical Moss Species

A. O. Adebiyi, P. O. Tedela
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引用次数: 1

Abstract

The crude extracts obtained from two tropical moss species namely: Philonotis hastata (Duby) Wijk & Margad and Barbula lambaranensis C. Mull were analysed with a view to identifying the bioactive compounds present in them. The mosses were collected from their natural population and air dried at ambient temperature in the laboratory for fourteen days. The aqueous extracts were subjected to phytochemical analysis using standard methods while the n- hexane extracts were subjected to gas chromatography-mass spectrometry (GC-MS) analysis. The results of the phytochemical screening showed the presence of alkaloids (0.189mg/g), saponins (0.047mg/g) and flavonoids (22.35QE/g) in P. hastata while the alkaloids, saponins and flavonoids content in B. lambaranensis were 0.184mg/g, 0.037mg/g and 14.18QE/g respectively. Steroids and reducing sugars were also present in the two moss species. The results of the GC-MS analysis showed the presence of 23 compounds in P. Hastate with 9-Octadecenoic acid methyl ester, 9,12-Octadecadienoic acid (Z,Z)- methyl ester, cis-Vaccenic acid, Hexadecanoic acid methyl ester and 1- Phenanthrenecarboxylic acid, tetradecahydro-7-(2-methoxy-2-oxoethlidene)-1,4a,8-trimethyl-9-oxo-, methyl ester forming the major components while in B. lambaranensis, 31 compounds were present from which Hexadecanoic acid methyl ester, 9,12-Octadecadienoic acid methyl ester, 13-Tetradecenal, 9-Octadecenoic acid (Z)- methyl ester and Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester formed the prominent components. Five compounds: Hexadecanoic acid methyl ester, n-Hexadecanoic acid, cis-Vaccenic acid, Octadecanoic acid and 9-Octadecanoic acid methyl ester were commonly identified in the two moss species. The identified compounds are considered to be biologically and pharmacologically important. Further investigation on the identified bioactive compounds from the studied moss species will be beneficial to formulate novel drugs for the treatment and management ...
两种热带苔藓植物化学特征及提取物的GC-MS分析
从两个热带苔藓物种即Philonotis hastata (Duby) Wijk & Margad和Barbula lambaranensis C. Mull中获得的粗提取物进行了分析,以确定其中存在的生物活性化合物。从自然种群中采集苔藓,在实验室环境温度下风干14天。水萃取物采用标准方法进行植物化学分析,正己烷萃取物采用气相色谱-质谱(GC-MS)分析。植物化学筛选结果显示,海苔中含有生物碱(0.189mg/g)、皂苷(0.047mg/g)和黄酮类化合物(22.35QE/g),而马齿苋中生物碱、皂苷和黄酮类化合物的含量分别为0.184mg/g、0.037mg/g和14.18QE/g。类固醇和还原糖也存在于两种苔藓中。气相色谱-质谱分析结果表明,海藻中含有23种化合物,其中9-十八烯酸甲酯、9,12-十八烯酸(Z,Z)-甲酯、顺式异戊酸、十六烯酸甲酯和1-菲二甲酸、十四氢-7-(2-甲氧基-2-氧乙基)-1,4a,8-三甲基-9-氧甲酯为主要成分;9,12-十八烯二酸甲酯,13-十四烯二醛,9-十八烯酸(Z)-甲酯和16酸,2-羟基-1-(羟甲基)乙酯是主要成分。在这两种苔藓中共鉴定出5种化合物:十六烷酸甲酯、正十六烷酸、顺式异丙酸、十八烷酸和9-十八烷酸甲酯。所鉴定的化合物被认为具有重要的生物学和药理学意义。进一步研究所研究苔藓物种中已鉴定的生物活性物质,将有助于研制新的治疗和管理药物。
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