Expressing activator protein Ap36 in Bacillus thuringiensis and the function of the recombined strain on disease resistance

Abstract

The ap36 gene from Alternaria spp. was sub-cloned into the downstream of slh (S-layer homology) motif of the pBMB982-304, the expressed vector with the fusion gene slh::fap was constructed and transformed into Bacillus thuringiensis plasmid-free derivative strain BMB171. Result showed that the SLH::Ap36 fusion protein was successful expressed. Tomato (Lycopersicum esculentum) chips treated with cultured recombinant strain at 28 ℃for 24 h , showed that the activity of peroxidase and the amount of proline of tomato leaves were increased 57.14% and 131.59%, respectively, compared to control after treated for 90 min,and the activity of phenylalanine ammonia lyase was also higher than that of control after treatment for 4 d. Furthermore, the treated potato (Solanum tuberosum)chips showed higher resistance to rot disease caused by Erwinia corotovora SCG1 compared to control.