Occurrence of Salmonella and Vibrio species in fresh fishes collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh and their characterization

Asian-Australasian Journal of Bioscience and Biotechnology Pub Date : 2018-04-28 DOI:10.3329/aajbb.v3i1.64752

A. Dutta, S. Kabir, Muhammad Tofazzal Hossain

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引用次数: 1

Abstract

This study was undertaken to determine total viable count (TVC), total salmonella count (TSC) and total vibrio count (TVibC) in fresh fishes collected from markets of Mymensingh, Gazipur and Sherpur districts. Isolation and identification of Salmonella spp. and Vibrio spp. was also done from pangas (n=20), tilapia (n=20) and koi (n=20) collected from different markets of Mymensingh, Gazipur and Sherpur districts. Samples were cultured on plate count agar to determine TVC, xylose-lysine deoxycholate agar (XLD) to determine TSC and thiosulfate citrate bile salt sucrose agar (TCBS) to determine TVibC. The mean value of logarithm colony forming units (CFU±SD/g) of TVC, TSC and TVibC in pangas were log 9.09±0.616, log 5.32±0.391 & log 3.14±0.557CFU/g; log 8.46±0.441, log 5.26±0.589 & log 3.59±0.823 CFU/g and log 7.58±0.466, log 3.28±0.493 & log 2.88±0.386 CFU/g of Mymensingh, Gazipur and Sherpur, respectively. Similarly, the mean values of TVC, TSC and TVibC in tilapia were log 6.60±0.790, log 3.59±0.388 & log 3.75±0.176 CFU/g; log 6.55±0.553, log 3.26±0.502 & log 3.67±0.021 CFU/g and log 6.74±0.372, log 3.44±0.411 & log 3.05±0.609 CFU/g of Mymensingh, Gazipur and Sherpur, respectively and in koi were log 7.51±0.537, log 3.49±0.459 & log 3.35±0.390 CFU/g; log 7.66±0.752, log 3.25±0.465 & log 3.59±0.581 CFU/g and log 7.13±0.393, log 3.27±0.384 & log 3.43±0.297 CFU/g of Mymensingh, Gazipur and Sherpur respectively. The targeted Vibrio spp. and Salmonella spp. were isolated and identified from collected fishes. All the isolates of Salmonella were confirmed by targeting genus specific histidine transport operon gene. Antimicrobial sensitivity test was done for all the isolates of Salmonella and Vibrio species by disc diffusion method. Out of forty five isolates of Salmonella, seven were found multidrug resistant. The bacteria isolated from fish were of public health significance as well as responsible for spoilage of fish. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 45-51

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从孟加拉国Mymensingh、Gazipur和Sherpur地区不同市场采集的鲜鱼中沙门氏菌和弧菌的发生情况及其特征

本研究旨在测定从Mymensingh、Gazipur和Sherpur地区市场采集的鲜鱼的总活菌数(TVC)、总沙门氏菌数(TSC)和总弧菌数(TVibC)。从Mymensingh、Gazipur和Sherpur地区不同市场采集的鲶鱼(n=20)、罗非鱼(n=20)和锦鲤(n=20)中分离鉴定沙门氏菌和弧菌。采用平板计数琼脂法测定TVC，木糖赖氨酸脱氧胆酸琼脂法测定TSC，硫代硫酸柠檬酸胆盐蔗糖琼脂法测定TVibC。pangas中TVC、TSC和TVibC的对数集落形成单位(CFU±SD/g)平均值分别为log 9.09±0.616、log 5.32±0.391和log 3.14±0.557CFU/g;Mymensingh、Gazipur和Sherpur分别为log 8.46±0.441、log 5.26±0.589和log 3.59±0.823 CFU/g和log 7.58±0.466、log 3.28±0.493和log 2.88±0.386 CFU/g。罗非鱼TVC、TSC和TVibC的平均值分别为log 6.60±0.790、log 3.59±0.388和log 3.75±0.176 CFU/g;Mymensingh, Gazipur和Sherpur的log分别为6.55±0.553,log 3.26±0.502和log 3.67±0.021 CFU/g和log 6.74±0.372,log 3.44±0.411和log 3.05±0.609 CFU/g, koi的log分别为log 7.51±0.537,log 3.49±0.459和log 3.35±0.390 CFU/g;Mymensingh、Gazipur和Sherpur的log分别为7.66±0.752、3.25±0.465和3.59±0.581 CFU/g和7.13±0.393、3.27±0.384和3.43±0.297 CFU/g。从所采集的鱼类中分离鉴定出针对性弧菌和沙门氏菌。所有分离株均以属特异性组氨酸转运操纵子基因为靶点进行鉴定。采用圆盘扩散法对分离的沙门氏菌和弧菌进行药敏试验。在45株沙门氏菌分离株中，有7株被发现具有多药耐药性。从鱼类中分离出的细菌具有公共卫生意义，同时也是导致鱼类变质的原因。亚洲Australas。j . Biosci。生物技术，2018,3 (1)，45-51

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Asian-Australasian Journal of Bioscience and Biotechnology

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