Inhibition of JAK/STAT Signaling Synergizes with CTLA4-Ig to Promote Transplant Survival

Abstract

Murine T cell activation was assessed through a CFSE proliferation assay. Activation in an inflamed environment was simulated through addition of supernatant (MATSup) from maturing dendritic cells. Jak inhibition was exerted with the inhibitor Tofacitinib (Tofa). Differential expression of DC maturation markers in response to LPS C/¡ Tofa was analyzed by flow cytometry. In vivo synergism between Tofa and CTLA4-Ig was tested in B6 to BALB/c heart and skin graft models.