In Vitro Micropropagation Studies Cotyledonary Explants Of Trichosanthes Anguina (L)

Abstract

A method for Invitro Micropropagation Through Cotyledonary Explants of Trichosanthes anguina was exercised from 10-15 days of young stage explants [1, 2]. Developed a protocol for cotyledonary explants cultures, callus induction, and regeneration through cotyledon callus tissue [3, 4]. Multiple shoot formation was promoted by BAP 1.0 mg/l to 3.0 to 5.0 mg/l and higher combination with NAA 3.0 to 4.0 mg/l Kn [5, 6]. Invitro produced small shoots were green callus induced to cotyledonary callus cultures green compact small shoots buds were induced in 16-20 days cultures respectively on Ms. Medium.