Real time simulation of heat detection in DNA Thermosequencing

Abstract

In this paper we study enzymatic and heat profile modeling for Thermosequencing [1-5], and discuss the real time 2D and 3D finite element simulation results for chemical concentrations involved in the detection system. The modeling simulates not only the heat released through the DNA polymerization reaction and its corresponding temperature change, but also the heat and sensing efficiencies of different geometries and platform structures. The thermodynamic effects of a DNA coated bead are investigated. Then, based on heat diffusion results, we recommend a modified gated structure for the microfluidic detection platform by using control valves and show how this new platform could dramatically improve the detection efficiency. Finally, we discuss potential advantages and disadvantages DNA Thermosequencing in real world applications.