Influence of culture media and environmental factors on mycelial growth, sporulation and spore germination behaviour of Curvularia eragrostidis (P. Hennings) Mayer

A. Saha, S Dasgupta, P. Mandal, D. Saha
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引用次数: 0

Abstract

Curvularia eragrostidis is a foliar fungal pathogen of young tea plants. It causes leaf spot disease of tea. Mycelial growth, sporulation and spore germination behaviour of the pathogen were studied. Six different media were tested for mycelial growth. Among these, potato carrot agar (PCA) was found best for the mycelial growth and sporulation. Maximum mycelial growth was attained after 15 days of incubation. Mycelial growth was also studied in different temperatures and pH. Optimum temperature of growth was 25 °C and best growth was obtained at pH 6.0. Glucose and peptone were best carbon and nitrogen sources respectively for growth and sporulation of the fungus. The optimum conditions of spore germination were found to be at pH 7.25 and at incubation temperature of 25 °C. Keywords: Sporulation; Curvularia eragrostidis; Mycelial growth, fungus
培养基和环境因素对曲霉菌丝生长、产孢和孢子萌发行为的影响
弯孢霉(Curvularia eragrostidis)是一种茶树叶面真菌致病菌。引起茶叶的叶斑病。对病原菌菌丝生长、产孢和孢子萌发行为进行了研究。对6种不同培养基进行菌丝生长试验。其中,马铃薯胡萝卜琼脂(PCA)对菌丝生长和产孢效果最好。培养15天后菌丝生长达到最大值。研究了菌丝在不同温度和pH下的生长情况,最适生长温度为25℃,pH为6.0时菌丝生长最佳。葡萄糖和蛋白胨分别是促进真菌生长和产孢的最佳碳源和氮源。在pH为7.25、培养温度为25℃的条件下,孢子萌发效果最佳。关键词:孢子形成;Curvularia eragrostidis;菌丝生长,真菌
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