Estimating Oligo-nucleotide Microarray Expression by Hybridization Process Modelling

Abstract

Oligonucleotide microarray technology has become widely spread in genetic probing for different purposes. This technology has shown to be highly reliable and dependable, although not free from problems regarding expression estimation, as it relies on the differential or contrasted hybridization of test probes. In many cases hybridization results do not show the expected behaviour, thus affecting to the reliability of expression estimation. In the present paper a modelling of the hybridization process is proposed which can be used to predict and correct expression levels on a specific test probe, improving the reliability of the results. Some examples from real microarray processing are shown and discussed