Establishment and evaluation of a transient expression method in Expi293F system for Fab monoclonal antibody fragment production

Abstract

Introduction: Monoclonal antibodies and their fragments produced by recombinant DNA technology represent a group of immunobiologicals with varied applications in immunotherapy and as a tool for immunodiagnostics assays development. Expression of these proteins require efficient cell culture systems that ensure agility in protein synthesis, acceptable benefit-cost ratios, high yields and expression of functional proteins for the desired applications.