Synthesis, Characterization and Size Evaluation of Biosynthesized Silver Nanoparticles by UV–Vis Spectroscopy

Abstract

Mie-Gans (MG) fitting model theoretical model utilizing the phenomena of scattering light to determine the morphologies, shape, and size of metallic nanoparticles in solution. In the present work, the average radius of biosynthesized silver nanoparticles (AgNPs) was evaluated based on the fitting of their Ultraviolet-visible (UV-Vis) spectra by the MG fitting model for spherical and non-spherical particles. Biosynthesis of AgNPs using Lemon (Citrus Limon) leaves extract as a reducing agent and Gum Acacia as a capping and stabilizing agent was studied for various concentrations of Citrus Limon leaves extract. The investigation of structural and optical properties was carried out for the synthesized samples using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and UV-Vis spectroscopy. XRD confirmed the structure of AgNPs and revealed that the structure of these nanoparticles was face-centered cubic (fcc). FTIR measurements indicate the presence of citric acid in Citrus Limon leaf extract which is responsible for reducing bioreduced AgNPs. UV-Vis spectroscopy determined the surface plasmon resonance (SPR) for AgNPs; the peaks of resonances of samples appear at 436-461nm range. MG fitting evaluations show that most of AgNPs were spherical in shape with an average radius in the range of 39-47nm. Moreover, this model allows the estimation of the fraction of nonspherical and aggregated AgNPs. These unique characteristics of AgNPs have made them applicable in a large number of fields like water treatment, biomedical, energy science, catalysis, etc.