Extracellular matrix: an immunological and biochemical (CAT and TOH activity) survey of in vitro differentiation of isolated amphibian neuroblasts

S. Huang, J.P. Saint-Jeannet, P. Kan, A.M. Duprat
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引用次数: 9

Abstract

After neural induction certain cells in the neuroepithelium immediately acquire the property to express certain neural phenotypes (Duprat et al., 1984, 1987). However, the activity of almost all the specific enzymes involved in the biosynthesis of neurotransmitters is considerably higher when neurectodermal cells are cultured with chordamesodermal cells than when they are cultured alone. The stimulating effects of chordamesoderm do not appear to be due to diffusible factors (Duprat et al., 1985b). The present study was designed to investigate the role of extracellular matrix components in neuronal cell differentiation. We showed that the extracellular matrix cannot replace chordamesoderm in stimulating the biochemical differentiation of neuroblasts, although fibronectin and especially laminin stimulate morphological differentiation. We suggest that interaction between neuronal and non-neuronal cells plays an important part in functional biochemical differentiation, whereas the molecules of extracellular matrix are important for morphological differentiation.

细胞外基质:分离的两栖类神经母细胞体外分化的免疫学和生化(CAT和TOH活性)调查
神经诱导后,神经上皮中的某些细胞立即获得表达某些神经表型的特性(Duprat et al., 1984,1987)。然而,当神经外胚层细胞与脊索内胚层细胞一起培养时,几乎所有参与神经递质生物合成的特定酶的活性都比单独培养时高得多。脊索皮层的刺激作用似乎不是由扩散因素引起的(Duprat et al., 1985b)。本研究旨在探讨细胞外基质成分在神经元细胞分化中的作用。我们发现细胞外基质不能取代脊索内胚层来刺激神经母细胞的生化分化,尽管纤维连接蛋白和层粘连蛋白可以刺激神经母细胞的形态分化。我们认为神经元细胞和非神经元细胞之间的相互作用在功能性生化分化中起重要作用,而细胞外基质分子在形态分化中起重要作用。
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