The anti-herpetic activity of fluorine-containing compounds based on β-D-glucopyranose

Abstract

The diseases caused by Herpes Simplex Virus 1 (HSV-1) are widely spread. The shortage of antiviral compounds due to their high toxicity and emergence of resistant viruses is a major problem in the treatment of patients. This work is related to the determination of the antiviral activity of new fluorine containing derivatives against HSV-1. Cytotoxicity and anti-herpetic activity of compounds 10S-25 (1-S-thio-(1methylsulfonyl-2difluoromethyl-vinyl)-2,3,4,6,-tetra-O-acetyl-β-D-glucopyranose) and 10S-27 1-(β-Dglucopyranosyl)-4-(hexafluoropropyl)-5-tosyl-1H-1,2,3-triazole) were studied using MTT assay. To sort the compounds, four experimental procedures were used: co-incubation of compounds and HSV-1, addition of compounds during virus adsorption and penetration, addition of compounds post-infection. The antiviral activity was assessed using real-time PCR and virus yield reduction assay. Compounds 10S-25 and 10S-27 demonstrated high toxicity for cells and their IC50 values were 13 and 250 μg/ml, respectively. It was determined, that only 10S-27 inhibited the formation CPE of the HSV-1 (EC50 value 48 μg/ml). The absence of virucidal activity and prevention of the adsorption and penetration of HSV-1 into cells were shown for this compound. But in the presence of 10S-27 in higher concentration, HSV-1 DNA replication was inhibited and the viral DNA copy number was reduced to 38 %. Moreover, it was found that 10S-27 in concentrations 4 150 μg/ml reduced the titer of the virus obtained de novo by 39 98%. Taken together, our results showed that 10S-27 possesses an anti-HSV-1 activity at non-toxic concentrations with multiple mechanisms, but further investigation is needed to explore this action in detail.