E. Candrawati, B. Rupaedah, S. Sumpono, A. Sundaryono
{"title":"KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT","authors":"E. Candrawati, B. Rupaedah, S. Sumpono, A. Sundaryono","doi":"10.29122/jbbi.v5i2.2769","DOIUrl":null,"url":null,"abstract":"Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis ","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29122/jbbi.v5i2.2769","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis
内生细菌活性复方提取物对尖孢镰刀菌生长的抑制作用。摘要油棕植物血管病是由尖孢镰刀菌引起的。这种疾病非常有害,因为它能够在不到一年的时间内杀死受感染的油棕植物。内生细菌可能是该病的生物控制者,因为它们能够产生生物活性抗真菌化合物。油棕植物内生细菌的分离及其对尖孢镰刀菌活性化合物的活性测定。体外实验已经完成。内生细菌分离株对尖孢镰刀菌的拮抗试验。采用双重培养法进行。用乙酸乙酯溶剂提取内生细菌分离物的活性成分,并对其抑菌活性进行了测试。结果表明,B11内生细菌活性化合物提取物在24和54小时的培养时间内对尖孢镰刀菌的生长有抑制作用。分别为29.23%和43.85%。关键词:拮抗试验;生物活性化合物;内生细菌;【摘要】油棕;油棕;油棕;Penyakit ini menjadi penyebab kematian tanaman kelapa看到yang telah terinfeksi dalam waktu kurang dari setahun。Bakteri endofit鼻tanaman kelapa sawwit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan Bakteri tersebut memproducksi senyawa bioaktif yang bersifat抗真菌药。白僵菌属植物的生长、生长、生长、生长和生长。体外丝瓜的培养。拮抗拮抗菌拮抗拮抗细菌拮抗细菌拮抗病菌。蒙古纳坎文化中心。分离孢杆菌对潜在的登革热、登革热、孟古纳罕、白藜芦醇等病原菌有拮抗作用,对登革热有拮抗作用,对登革热有拮抗作用。Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp。Sebesar - masing-masing 29,23% Dan 43,85%。Kata Kunci:芽孢杆菌;, kelapa sawwit, senyawa aktif, uji antagonis