Pattern of Susceptibility Antibiotics against Clinical Isolates of Conjunctivitis from Sudanese Patients in Khartoum State

Abstract

Resistant Antibiotics of bacterial conjunctivitis is a major challenge globally. The study aimed to determine the most common bacterial agents and risk factors, associated with Conjunctivitis among Sudanese patients and  the sensitivity of the antibiotics used against isolates bacteria. The study was cross-sectional and carried out in MAKKA  and Allwaledin Eye Hospital over three months in 2019. Fifty samples were collected from patients suffer from conjunctivitis by fine swab. Isolates were cultured in different media agar media and biochemical tests were used for identifications. The demographic data was collected by a questionnaire filled by the patient after verbal consent. Antibiotic susceptibility testing was conducted by the disc diffusion method (Kirby-Bauer) was performed as recommended by the CLSI. Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC27853) were used as controls. The positive frequency samples were 38 (76%), and the negative samples were 12(24%). Among the 38 positive samples 19(50%) were females while 19(50%) were males. The difference was not significant P= 0.241) with highly infected age groups (1-10) years old. Our Results detected that Some Isolated bacteria developed resistance to certain antibiotics, such as Staphylococcus aureus exhibited resistance against Erythromycin 10/15 (66.7%).While Staphylococcus epidermidis resistant against Tetracycline, and Erythromycin 4/4 (100%). Also, Bacillus spp revealed resistance against Ceftazidime1/1(100%). Listeria monocytogenes was resistant against Ceftazidime and Erythromycin 1/1(100%).On the other hand Pseudomonas aeruginosa, klebsiella pneumonia and klebsiella oxytoca exhibited resistance against Amoxicillin clavulanic acid and Erythromycin. The record data of risk factors  conducted by highest frequency include antibiotics (16(36%); P=0.874) and using makeup and viral infection 11(22%); P=0.142) followed by eye surgery, arthritis 6 (12%); P=0.145), diabetes mellitus and hypertension 5(13.2%) ; P=0.654), using the immunosuppressive drug, lens and eye injury 4(8%) ; P=0.654), Smoking (3(6%) ; P=0.961) and heart disease 1(2%) ; P=0.322) associated with conjunctivitis statistically in significant.  According to the results, we obtained that the main bacteria that caused conjunctivitis were Staphylococcus aureus and Pseudomonas aeruginosa. The sensitivity test indicated that Ciprofloxacin, Ceftazidime and Gentamycin were the most effective than other drugs used against isolates identified in this study.