Phytochemical and Antibacterial Study of Ethanol and Ethyl acetate Extracts from Leaves of Alchornea Cordifolia Against Isolates from Infected Wounds

Abstract

Alchornea cordifolia leaves have been reported to be used traditionally to treat wound infections. The leaves ofAlchornea cordifolia were extracted with ethanol which was partitioned with ethyl acetate to obtain the ethanolextract and ethyl acetate fraction respectively. The extracts were screened for basic phytocompounds and subjectedto antibacterial assay against bacterial isolates from infected wounds which include Pseudomonas aeruginosa,Staphylococcus aureus, Klebsiella spp, Enterobacter spp, Citrobacter spp, Escherichia coli, Proteus mirabilis andProteus vulgaris. Agar well diffusion method was used to determine the antibacterial activity of the extract andfraction. Phytochemical investigation revealed the presence of carbohydrates, cardiac glycosides, saponins,flavonoids, tannins and alkaloids. The zone of inhibition ranged between 1.0 - 32.2mm for the entiremicroorganisms. This variation in level of the activity among the extracts could be due to the difference in solubilityof the active ingredient in each solvent on one hand and to the constitutional or structural variability of the testedorganisms on the other hand. The results of the study showed that the ethyl acetate fraction of Alchornea cordifolialeaves possess high level of antibacterial activity against bacteria associated with wound infection. This givescredence to traditional medicine application of the plant for treating wound infections.