Assessing the efficacy of molecularly targeted agents by using Kalman filter

Abstract

A novel preclinical model combining experimental methods and theoretical analysis is proposed to investigate the mechanism of action and identify pharmacodynamic characteristic of a drug. Instead of fixed time point analysis of the drug exposure to drug effect, the time course of drug effect for different doses are quantitatively studied on cell line-based platforms using Kalman filter, where tumor cells' responses to drugs through the use of fluorescent reporters are sampled frequently over a time-course. It is expected that such preclinical study will provide valuable suggestions about dosing regimens for in vivo experimental stage to increase productivity.