Total Phenolic Content, Antioxidant and Antityrosinase Activities of Garcinia dulcis Root Extract

Abstract

The objective of this study is to investigate total phenolic content, antioxidant and antityrosinase activities of G.dulcis root extract and fractions (A-J). It found that the total phenolic content of the crude extract was 338.42 ± 6.08 mg GAE/g extract. The extract showed good activity against DPPH (IC50 71.07 ± 0.02 μg/ml), ABTS (IC50 28.08 ± 0.01 μg/ml) and good ferric reducing antioxidant power (235.40 ± 3.44 mg AAE/g extract). It also showed inhibitory activity toward tyrosinase enzyme (IC50 354.15 ± 0.13 μg/ml). Fractions E and I showed better activity than the extract and other fractions in all tested. 12b-hydroxy-des-D-garcigerin A and globuxanthone are major components from fraction E and symphoxanthone was obtained from fraction I. The result indicated that fraction from G.dulcis root extract is a natural antioxidant and antityrosinase source with potential for use as active ingredients in cosmetics.