[Cytotoxicity evaluation of root canal sealers by fibroblast culture].

Abstract

This study was undertaken to evaluate the cytotoxic effects of 8 root canal sealers. Fibroblasts of human gingiva were employed to determine the comparative cytotoxicity of the 8 experimental materials. The results of this experiment were analyzed by counting the cells on the 2nd, 4th and 7th day. The results were as follows: 1. All experimental groups exhibited significant decrease of cell numbers in compared with control. All experimental groups showed cytotoxicity at 2, 4, 7 days (P less than 0.05). 2. All experimental groups except AH26 exhibited the decrease of mean of cell numbers with increasing time. 3. On the 7th day, AH26' Tubli-Seal and NOgenol showed lower cytotoxic effect than Apatite root sealer (Type I, II, III), N2 universal and Sealapex. 4. There was no statistical significance of cytotoxicity among the experimental groups (Type I, II, III) of Apatite root sealer (P greater than 0.05).