The study of antigenotoxic activity of the medicinal plants infusions of trans-Ili Alatau

Abstract

Due to growing number of factors causing a hazard effect to nature, the search for protectors becomes urgent in environment. Using the bioluminescent test (lux-biosensor), genotoxic, oxidative, antigenotoxic and antioxidant activities of sage (Salvia officinalis), oregano (Origanum vulgare), chamomile(Matricaria chamomilla) and yarrow (Achillea millefolium) have been studied. We used genetically modified E. coli strains: E. coli MG 1655 (pColD-lux), E. coli MG 1655 (pRecA-lux), E. coli MG 1655 (pSoxSlux), E. coli MG 1655 (pKatG-lux). The operon MG 1655 is responsible for the work on luciferase andthe provision of bioluminescence, which applied in this test for its reporter function. The infusions investigated under various methods of preparation (concentrated, diluted and phyto-tea) did not reveal genotoxic and oxidative activity. The induction factor of the SOS-response in all strains is statistically significant, not exceeding the level of the negative control (distilled water). The combined effect of medicinalplants with mutagen 4-nitroquinoline 1-oxide (4-NQO) and oxidants paraquat with hydrogen peroxidedid not show a statistically significant decrease in SOS responses to the pColD-lux, pRecA-lux, pKatGlux pSoxS-lux sensors, induced by 4-NQO, hydrogen peroxide and paraquat. The exception alertedby sage. Concentrated sage infusion, prepared according to the recipe, and sage phyto-tea statisticallysignificantly reduce the induction factor of the pKatG-lux biosensor SOS response (p <0.001). The levelof inhibition depended on the type of infusion. Concentrated infusion and phyto-tea showed a strongantioxidant effect against hydrogen peroxide, while inhibition was 43.6% and 46.8%, respectively. Diluted sage infusion showed a moderate antioxidant effect with an inhibition rate of 29.2%. Thus, using abioluminescent test, antioxidant activity of the concentrated infusion and phyto-tea of sage are releasedusing the pKatG-lux biosensor. It can be assumed, that the sage infusion contains biologically activesubstances that are capable of both inactivating hydrogen peroxides and organic peroxides. Consideringthat oregano, chamomile and yarrow contain many biologically active substances, but the test did notreveal antigenotoxic and antioxidant activity. Therefore, it can be concluded that the required amount ofbiologically active substances for detect activity is not extracted during the preparation of the infusion.Key words: lux-biosensors, Salvia, Origanum, Matricaria, Achillea, antioxidant.