Single Cell Manipulation and Characterization Intended to Study Pathology of Neural Diseases

Abstract

Organ-on-chip opened the gate to study human diseases in vitro, especially brain disorders. In this work, we designed a cell manipulation chip based on a microelectrodes array (MEA) for precisely localizing one single cell in a specific position. Dielectrophoresis, created by nonuniform electrical field, was used to attract cells onto microelectrodes. Every microelectrode is intended to manipulate cells independently and record signals from selected neural or muscle cells. Network of neurons could be developed on the proposed MEA chip, with precisely recording and continuously monitoring, to study the pathology of human neural diseases.