Evaluation of the efficiency of polysulfone ultrafiltration membrane-based water purifiers for microbiological decontamination

V. Nagar, R. Shashidhar, Arun Sharma, J. Bandekar, R. C. Bindal, S. Prabhakar, P. K. Tewari
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Abstract

Indigenously developed polysulfone Ultrafiltration (UF) membrane-based domestic and industrial water purification units were evaluated for their ability to filter bacteria and viruses from water. Escherichia coli (105 cfu/ml) and P1 phage (106 pfu/ml) were filtered through a Domestic Water Purifier (DWP) (dead-end UF unit) and a large-scale spiral water purifier (cross-flow UF unit) and the filtrates were analysed for bacterial and phage counts. Both units were found to be efficient in the complete removal of E. coli and a 99.99% removal of the P1 phage was observed. Both the domestic and industrial water purifiers are highly efficient in the removal of bacteria and viruses. UF membranes were also characterised for Molecular Weight Cut-Off (MWCO) by the polyethylene glycol and polyethylene oxide methods, which showed that MWCO was 60–65 kDa. These MWCO results were further validated using the Bovine Serum Albumin (BSA) rejection behaviour of these membranes, which showed about 90% rejection. The technique involving the estimation of MWCO, coupled with the rejection characteristics of E. coli and P1 phage, provides an excellent tool to evaluate the efficiency of water purifiers based on UF membrane technology.
聚砜超滤膜净水器微生物去污效能评价
对国产聚砜超滤(UF)膜为基础的家用和工业净水装置对水中细菌和病毒的过滤能力进行了评价。大肠杆菌(105 cfu/ml)和P1噬菌体(106 pfu/ml)分别通过家用净水器(DWP)(终端UF装置)和大型螺旋净水器(交叉流UF装置)过滤,并对滤液进行细菌和噬菌体计数分析。这两种装置都能有效地完全去除大肠杆菌,并观察到P1噬菌体的去除率为99.99%。家用和工业用净水器都能高效去除细菌和病毒。用聚乙二醇和聚氧化物对超滤膜进行了分子量切断(MWCO)表征,MWCO为60-65 kDa。利用这些膜的牛血清白蛋白(BSA)排斥行为进一步验证了这些MWCO结果,其排斥率约为90%。该技术结合大肠杆菌和P1噬菌体的拒斥特性,为UF膜技术净水器的效率评估提供了一个很好的工具。
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